Hotspot

trnR-UCU-atpA

LSC · 37728-38215 · 488 bp

Back to Hovenia

Pi 0.0082

MarkerSeek score 49.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 6
pi: 0.0082
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GTTGGAATGAAAAGCGTCCA	AGGAACAGCTGGAACGTTTT	45.0	45.0	56.9	57.9	614-690 bp	1.0	72.0
trnR-UCU-atpA_p2	GTTGGAATGAAAAGCGTCCA	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	56.9	58.1	648-724 bp	1.0	71.7
trnR-UCU-atpA_p3	GTTGGAATGAAAAGCGTCCA	TTTACCGAGGAAGCAGAAGC	45.0	50.0	56.9	57.9	654-730 bp	1.0	71.6
trnR-UCU-atpA_p4	GTTGGAATGAAAAGCGTCCA	ATTCAGGAACAGCTGGAACG	45.0	50.0	56.9	58.2	618-694 bp	1.0	70.9
trnR-UCU-atpA_p5	AGTTGGAATGAAAAGCGTCC	AGGAACAGCTGGAACGTTTT	45.0	45.0	56.6	57.9	615-691 bp	1.0	69.6