Hotspot

trnS-GCU-trnR-UCU

LSC · 34900-37655 · 2756 bp

Back to Hovenia

Pi 0.00701

MarkerSeek score 45.30

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 2756
variable_sites: 29
pi: 0.00701
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	2827-2863 bp	1.0	66.1
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	2826-2862 bp	1.0	66.1
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	2833-2869 bp	1.0	65.5
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	2832-2868 bp	1.0	65.5
trnS-GCU-trnR-UCU_p5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	2881-2917 bp	1.0	64.9