Hotspot

matK-trnK-UUU

LSC · 30151-30900 · 750 bp

Back to Hovenia

Pi 0

MarkerSeek score 59.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCACGATCATGAGCAAATGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	880-883 bp	1.0	78.0
matK-trnK-UUU_p2	CCACGATCATGAGCAAATGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	879-882 bp	1.0	78.0
matK-trnK-UUU_p3	CCACGATCATGAGCAAATGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.9	57.5	893-896 bp	1.0	75.7
matK-trnK-UUU_p4	CCACGATCATGAGCAAATGC	TTCGGGTTGCTAACTCAACG	50.0	50.0	57.9	58.5	896-899 bp	1.0	75.4
matK-trnK-UUU_p5	AGGAGATCGTGTTGCTGAGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.4	58.2	828-831 bp	1.0	75.3