Hotspot

ycf1

SSC · 125314-130632 · 5319 bp

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Pi 0.00161
MarkerSeek score 46.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5319
variable_sites
46
pi
0.00161
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CACGCTTGCCTTTTCTTGAA CGTTCGACGTTAGCAAGGAA 45.0 50.0 57.5 58.6 324 bp 1.0 73.3
ycf1_p2 ACGCTTGCCTTTTCTTGAAC CGTTCGACGTTAGCAAGGAA 45.0 50.0 57.5 58.6 323 bp 1.0 73.3
ycf1_p3 CACACGCTTGCCTTTTCTTG CGTTCGACGTTAGCAAGGAA 50.0 50.0 58.5 58.6 326 bp 1.0 73.1
ycf1_p4 ATCCTCCGTTGATTCCTCGT CGTTCGACGTTAGCAAGGAA 50.0 50.0 58.5 58.6 293 bp 0.923077 69.2
ycf1_p5 CTCCGTTGATTCCTCGTCAT CGTTCGACGTTAGCAAGGAA 50.0 50.0 57.4 58.6 290 bp 0.923077 68.6