Hotspot

rps3

LSC · 83710-84372 · 663 bp

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Pi 0.00159
MarkerSeek score 46.20
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
663
variable_sites
6
pi
0.00159
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 CTATCACTGGACAGAACGGC ACTTGTTTAGAGAAGAGAAATCTCA 55.0 32.0 58.1 55.9 810-815 bp 1.0 41.3
rps3_p2 CTATCACTGGACAGAACGGC AACTTGTTTAGAGAAGAGAAATCTCA 55.0 30.8 58.1 56.6 811-816 bp 1.0 41.3
rps3_p3 CTATCACTGGACAGAACGGC ACTTGTTTAGAGAAGAGAAATCTCAA 55.0 30.8 58.1 56.6 810-815 bp 1.0 41.3
rps3_p4 CTATCACTGGACAGAACGGC CTTGTTTAGAGAAGAGAAATCTCA 55.0 33.3 58.1 54.3 809-814 bp 1.0 41.3
rps3_p5 CTATCACTGGACAGAACGGC ACTTGTTTAGAGAAGAGAAATCTC 55.0 33.3 58.1 54.3 810-815 bp 1.0 41.3