Hotspot

ndhA

SSC · 121144-123331 · 2188 bp

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Pi 0.00227
MarkerSeek score 51.00
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2188
variable_sites
22
pi
0.00227
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 CCGGTCACCATAGGGAACAT GTCCACCGGGTTTCATCAAT 55.0 50.0 59.2 58.2 2423-2438 bp 1.0 70.2
ndhA_p2 CGGTCACCATAGGGAACATA GTCCACCGGGTTTCATCAAT 50.0 50.0 56.4 58.2 2422-2437 bp 1.0 66.8
ndhA_p3 CCGGTCACCATAGGGAACAT TCCACCGGGTTTCATCAATT 55.0 45.0 59.2 57.4 2422-2437 bp 1.0 66.8
ndhA_p4 CCGGTCACCATAGGGAACA GTCCACCGGGTTTCATCAAT 57.9 50.0 58.7 58.2 2423-2438 bp 1.0 65.8
ndhA_p5 CCGGTCACCATAGGGAACAT CCGGGTTTCATCAATTTGCA 55.0 45.0 59.2 57.3 2418-2433 bp 1.0 65.6