Hotspot

rps15-ycf1

SSC · 124905-125313 · 409 bp

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Pi 0.00558
MarkerSeek score 47.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
409
variable_sites
9
pi
0.00558
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCCCCGTATCTTATCGGTGA ACGGCAGTCATTTCAGTATGT 50.0 42.9 57.3 57.7 565-570 bp 1.0 66.0
rps15-ycf1_p2 AGTCCCCGTATCTTATCGGT ACGGCAGTCATTTCAGTATGT 50.0 42.9 57.3 57.7 567-572 bp 1.0 65.9
rps15-ycf1_p3 GTCCCCGTATCTTATCGGTG ACGGCAGTCATTTCAGTATGT 55.0 42.9 57.3 57.7 566-571 bp 1.0 65.5
rps15-ycf1_p4 AAGTCCCCGTATCTTATCGGT ACGGCAGTCATTTCAGTATGT 47.6 42.9 58.0 57.7 568-573 bp 1.0 62.9
rps15-ycf1_p5 TCCCCGTATCTTATCGGTGAA ACGGCAGTCATTTCAGTATGT 47.6 42.9 58.1 57.7 565-570 bp 1.0 62.9