Hotspot

rpl32-trnL-UAG

SSC · 114870-115531 · 662 bp

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Pi 0.0137
MarkerSeek score 62.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
662
variable_sites
28
pi
0.0137
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAGTCAGTTTCTACCGG AGCGTGTCTACCGATTTCAC 47.6 50.0 58.2 58.0 767-783 bp 1.0 73.5
rpl32-trnL-UAG_p2 AGCAAAGTCAGTTTCTACCGG CAGCGTGTCTACCGATTTCA 47.6 50.0 58.2 58.0 768-784 bp 1.0 73.5
rpl32-trnL-UAG_p3 GCAAAGTCAGTTTCTACCGGA AGCGTGTCTACCGATTTCAC 47.6 50.0 58.2 58.0 766-782 bp 1.0 73.5
rpl32-trnL-UAG_p4 GCAAAGTCAGTTTCTACCGGA CAGCGTGTCTACCGATTTCA 47.6 50.0 58.2 58.0 767-783 bp 1.0 73.4
rpl32-trnL-UAG_p5 GCAAAGTCAGTTTCTACCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 56.7 58.0 766-782 bp 1.0 73.3