Hotspot

psbE-petL

LSC · 65477-66696 · 1220 bp

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Pi 0.00252
MarkerSeek score 50.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1220
variable_sites
11
pi
0.00252
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AGCAGCCAATAGAAAACCGA 50.0 45.0 57.9 57.5 1361-1370 bp 1.0 76.7
psbE-petL_p2 ACTGACGAATAACCAACCCG AGCAGCCAATAGAAAACCGA 50.0 45.0 57.9 57.5 1374-1383 bp 1.0 76.4
psbE-petL_p3 ACGAATAACCAACCCGCAAT AGCAGCCAATAGAAAACCGA 45.0 45.0 58.2 57.5 1370-1379 bp 1.0 75.8
psbE-petL_p4 CGAATAACCAACCCGCAATG AGCAGCCAATAGAAAACCGA 50.0 45.0 57.8 57.5 1369-1378 bp 1.0 75.6
psbE-petL_p5 ACCAACCCGCAATGAATAGG AGCAGCCAATAGAAAACCGA 50.0 45.0 58.2 57.5 1363-1372 bp 1.0 75.2