Hotspot

petA-psbJ

LSC · 63594-64707 · 1114 bp

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Pi 0.00143
MarkerSeek score 54.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1114
variable_sites
7
pi
0.00143
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.8 58.3 1245-1257 bp 1.0 77.0
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 57.8 57.6 1241-1253 bp 1.0 76.0
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 57.8 57.6 1244-1256 bp 1.0 76.0
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC CGGTTCATATTCCGGGTTGG 50.0 55.0 57.8 58.7 1246-1258 bp 1.0 73.9
petA-psbJ_p5 GGCCTTTTGTTCTTCTTGGC TATTCCGGGTTGGGTTCATC 50.0 50.0 57.8 57.3 1239-1251 bp 1.0 73.7