Hotspot

rbcL-accD

LSC · 57210-57946 · 737 bp

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Pi 0.00135
MarkerSeek score 32.80
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
737
variable_sites
4
pi
0.00135
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 ACTACAGTTCGGTGGAGGAA CACCAGTATGTCCAACAGCA 50.0 50.0 58.0 57.8 1089-1102 bp 1.0 79.7
rbcL-accD_p2 AATGAGGGACGTGATCTTGC CACCAGTATGTCCAACAGCA 50.0 50.0 58.0 57.8 992-1005 bp 1.0 79.7
rbcL-accD_p3 GGCTTCAGGGGGTATTCATG CACCAGTATGTCCAACAGCA 55.0 50.0 58.0 57.8 1155-1168 bp 1.0 79.6
rbcL-accD_p4 GAAGGGGAACGTGAGATGAC CACCAGTATGTCCAACAGCA 55.0 50.0 58.0 57.8 1280-1293 bp 1.0 79.5
rbcL-accD_p5 GATCTTGCTCGCGAAGGTAA CACCAGTATGTCCAACAGCA 50.0 50.0 58.1 57.8 980-993 bp 1.0 79.3