Hotspot

atpH-atpI

LSC · 13377-13874 · 498 bp

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Pi 0.00154
MarkerSeek score 50.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
498
variable_sites
3
pi
0.00154
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT ATAGGGGAATCCATGGAGGG 45.0 55.0 58.0 57.9 546-563 bp 1.0 82.5
atpH-atpI_p2 CGCAATACCTTCTACGGCTT ATAGGGGAATCCATGGAGGG 50.0 55.0 58.1 57.9 634-651 bp 1.0 81.8
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.0 57.9 571-588 bp 1.0 81.8
atpH-atpI_p4 TACCTTGACCAACTCCAGGT ATAGGGGAATCCATGGAGGG 50.0 55.0 57.9 57.9 602-619 bp 1.0 81.6
atpH-atpI_p5 CGCAATACCTTCTACGGCTT TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.1 57.9 659-676 bp 1.0 81.2