Hotspot

ndhF

SSC · 111015-113240 · 2226 bp

Back to Horsfieldia

Pi 0.00666

MarkerSeek score 37.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 27
pi: 0.00666
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAGCGACAGAAGTCTTGAG	TGCTCCTTCCGAAAGAGAGA	55.0	50.0	58.0	58.1	2846-2868 bp	1.0	80.5
ndhF_p2	GGGCCTTACAATCACTAGCC	TGCTCCTTCCGAAAGAGAGA	55.0	50.0	58.0	58.1	2551-2573 bp	1.0	80.5
ndhF_p3	GGGGCCTTACAATCACTAGC	TGCTCCTTCCGAAAGAGAGA	55.0	50.0	58.0	58.1	2552-2574 bp	1.0	80.5
ndhF_p4	CCTTGTTCCTACATGACCCG	TGCTCCTTCCGAAAGAGAGA	55.0	50.0	58.0	58.1	2715-2737 bp	1.0	80.4
ndhF_p5	GTCAAGTGGAAGTGCAGTGA	TGCTCCTTCCGAAAGAGAGA	50.0	50.0	58.0	58.1	2776-2798 bp	1.0	80.2