Hotspot

trnN-GUU-trnR-ACG

SSC · 130719-131346 · 628 bp

Back to Horsfieldia

Pi 0.0317

MarkerSeek score 58.10

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: SSC
length_bp: 628
variable_sites: 36
pi: 0.0317
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	GGGCCTTACAATCACTAGCC	55.0	55.0	58.0	58.0	1195-1210 bp	1.0	84.5
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GGGGCCTTACAATCACTAGC	55.0	55.0	58.0	58.0	1196-1211 bp	1.0	84.5
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	CCTTGTTCCTACATGACCCG	55.0	55.0	58.0	58.0	1359-1374 bp	1.0	84.0
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	GTCAAGTGGAAGTGCAGTGA	55.0	50.0	58.0	58.0	1420-1435 bp	1.0	83.7
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGCATCCTAACAG	55.0	55.0	58.0	58.0	1394-1409 bp	1.0	83.7