Hotspot

petA-psbJ

LSC · 65637-66834 · 1198 bp

Back to Horsfieldia

Pi 0.0132

MarkerSeek score 63.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 30
pi: 0.0132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCATCTGTTATTCTGGCA	CTGTGATCGGTTCAATGGGT	45.0	50.0	57.2	57.9	1349-1354 bp	1.0	74.5
petA-psbJ_p2	TGTCCAGGGTTTGTTGTTCT	CTGTGATCGGTTCAATGGGT	45.0	50.0	57.2	57.9	1372-1377 bp	1.0	74.4
petA-psbJ_p3	GTGTCCAGGGTTTGTTGTTC	CTGTGATCGGTTCAATGGGT	50.0	50.0	57.1	57.9	1373-1378 bp	1.0	74.0
petA-psbJ_p4	CGTGTCCAGGGTTTGTTGTT	CTGTGATCGGTTCAATGGGT	50.0	50.0	58.9	57.9	1374-1379 bp	1.0	73.7
petA-psbJ_p5	TTGGCATCTGTTATTCTGGCA	CTGTGATCGGTTCAATGGGT	42.9	50.0	57.9	57.9	1350-1355 bp	1.0	72.3