Hotspot

atpB-rbcL

LSC · 57105-57866 · 762 bp

Back to Horsfieldia

Pi 0.0059

MarkerSeek score 65.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 8
pi: 0.0059
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTTCAAGTGTGGAAACCCCA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	838-860 bp	1.0	74.7
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	899-921 bp	1.0	74.5
atpB-rbcL_p3	CTTCAAGTGTGGAAACCCCA	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	837-859 bp	1.0	74.4
atpB-rbcL_p4	GGGGGAAAAGCTACATCCAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	898-920 bp	1.0	74.2
atpB-rbcL_p5	AAACCCCAGGACCAGAAGTA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	826-848 bp	1.0	74.2