Hotspot

ndhC-trnV-UAC

LSC · 52243-54143 · 1901 bp

Back to Horsfieldia

Pi 0.0154
MarkerSeek score 49.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1901
variable_sites
57
pi
0.0154
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TAGGGGCTAAAACTCCGGAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 2031-2034 bp 1.0 81.3
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 2029-2032 bp 1.0 80.6
ndhC-trnV-UAC_p3 ATAGGGGCTAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 2032-2035 bp 1.0 80.2
ndhC-trnV-UAC_p4 TAGGGGCTAAAACTCCGGAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 2030-2033 bp 1.0 78.6
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 2028-2031 bp 1.0 77.8