Hotspot

trnE-UUC-trnT-GGU

LSC · 32492-33025 · 534 bp

Back to Horsfieldia

Pi 0.00655

MarkerSeek score 52.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 7
pi: 0.00655
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	637-687 bp	1.0	78.7
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GACTGAAGACTGGAGCTTCG	55.0	55.0	58.3	58.0	681-731 bp	1.0	78.6
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	616-666 bp	1.0	78.6
trnE-UUC-trnT-GGU_p4	CGTAGTACCCTACCTACCCC	GAACCGATGACTTACGCCTT	60.0	50.0	57.7	58.0	676-726 bp	1.0	78.5
trnE-UUC-trnT-GGU_p5	CGTAGTACCCTACCTACCCC	GACTGAAGACTGGAGCTTCG	60.0	55.0	57.7	58.0	720-770 bp	1.0	78.5