Hotspot

psbM-trnD-GUC

LSC · 30815-31807 · 993 bp

Back to Horsfieldia

Pi 0.00822

MarkerSeek score 51.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 15
pi: 0.00822
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1103-1104 bp	1.0	78.3
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1102-1103 bp	1.0	77.4
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1104-1105 bp	1.0	76.6
psbM-trnD-GUC_p4	AGAATGAACAGCGCAGTAGC	AGTTCAATCGGTCAGAGCAC	50.0	50.0	58.6	57.9	1106-1107 bp	1.0	75.3
psbM-trnD-GUC_p5	ACTAGAATGAACAGCGCAGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.2	57.9	1109-1110 bp	1.0	74.4