Hotspot

petN-psbM

LSC · 29835-30709 · 875 bp

Back to Horsfieldia

Pi 0.0223

MarkerSeek score 59.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 36
pi: 0.0223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1034-1039 bp	1.0	81.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1035-1040 bp	1.0	80.3
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.6	58.3	973-978 bp	1.0	80.0
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	50.0	45.0	58.2	58.5	1033-1038 bp	1.0	79.5
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.6	57.6	974-979 bp	1.0	79.0