Hotspot

trnK-UUU-rps16

LSC · 4181-5155 · 975 bp

Back to Horsfieldia

Pi 0.0104

MarkerSeek score 55.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 17
pi: 0.0104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	TCTTTTTCGAGCCGTACGAG	55.0	50.0	58.0	58.0	1065-1278 bp	1.0	84.9
trnK-UUU-rps16_p2	ACACATCTGTATCCACCCCA	TCTTTTTCGAGCCGTACGAG	50.0	50.0	58.0	58.0	1415-1630 bp	1.0	84.6
trnK-UUU-rps16_p3	GGAGTCCAATCAACGGATCC	TCTTTTTCGAGCCGTACGAG	55.0	50.0	58.0	58.0	1566-1781 bp	1.0	84.4
trnK-UUU-rps16_p4	GGGAGTCCAATCAACGGATC	TCTTTTTCGAGCCGTACGAG	55.0	50.0	58.0	58.0	1567-1782 bp	1.0	84.4
trnK-UUU-rps16_p5	ACCCGATCGCTCTTTTGATC	TCTTTTTCGAGCCGTACGAG	50.0	50.0	58.1	58.0	1468-1683 bp	1.0	84.4