Hotspot

trnL-CAA-ycf15

IRa · 132018-132659 · 642 bp

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Pi 0.00187
MarkerSeek score 47.70
Primer available yes
Label trnL-CAA-ycf15

Feature Metadata

label_name
trnL-CAA-ycf15
feature_type
igs
region
IRa
length_bp
642
variable_sites
3
pi
0.00187
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf15_p1 AAATGGTAGACACGCGAGAC GCCAAAGTCACTGAATGGGT 50.0 50.0 58.0 58.4 825 bp 1.0 77.2
trnL-CAA-ycf15_p2 AAATGGTAGACACGCGAGAC TGCCAAAGTCACTGAATGGG 50.0 50.0 58.0 58.4 826 bp 1.0 77.2
trnL-CAA-ycf15_p3 TGAAATGGTAGACACGCGAG GCCAAAGTCACTGAATGGGT 50.0 50.0 58.0 58.4 827 bp 1.0 77.2
trnL-CAA-ycf15_p4 TGAAATGGTAGACACGCGAG TGCCAAAGTCACTGAATGGG 50.0 50.0 58.0 58.4 828 bp 1.0 77.2
trnL-CAA-ycf15_p5 TGGTGAAATGGTAGACACGC GCCAAAGTCACTGAATGGGT 50.0 50.0 58.2 58.4 830 bp 1.0 75.7