Hotspot

rpl32-trnL-UAG

SSC · 106072-106776 · 705 bp

Back to Hordeum

Pi 0.011

MarkerSeek score 46.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 705
variable_sites: 20
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	817-851 bp	1.0	85.0
rpl32-trnL-UAG_p2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	820-854 bp	1.0	84.5
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	789-823 bp	1.0	84.4
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	788-822 bp	1.0	84.4
rpl32-trnL-UAG_p5	TTCCAGCGGTAATGAGCATC	GATCCGCACAAAAGAGTTGC	50.0	50.0	58.0	58.0	1363-1397 bp	1.0	83.0