Hotspot

ndhF-rpl32

SSC · 105003-105879 · 877 bp

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Pi 0.0122

MarkerSeek score 54.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 877
variable_sites: 24
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	55.0	28.6	58.1	52.2	918-961 bp	1.0	46.1
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	55.0	28.6	58.1	52.2	919-962 bp	1.0	46.1
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	55.0	28.6	58.1	52.2	920-963 bp	1.0	46.1
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	55.0	29.2	58.1	55.3	945-988 bp	1.0	46.0
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	55.0	28.6	58.1	52.2	921-964 bp	1.0	46.0