Hotspot

ycf15-trnL-CAA

IRb · 85502-86143 · 642 bp

Back to Hordeum

Pi 0.00187
MarkerSeek score 47.70
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
642
variable_sites
3
pi
0.00187
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 GCCAAAGTCACTGAATGGGT AAATGGTAGACACGCGAGAC 50.0 50.0 58.4 58.0 825 bp 1.0 77.2
ycf15-trnL-CAA_p2 TGCCAAAGTCACTGAATGGG AAATGGTAGACACGCGAGAC 50.0 50.0 58.4 58.0 826 bp 1.0 77.2
ycf15-trnL-CAA_p3 GCCAAAGTCACTGAATGGGT TGAAATGGTAGACACGCGAG 50.0 50.0 58.4 58.0 827 bp 1.0 77.2
ycf15-trnL-CAA_p4 TGCCAAAGTCACTGAATGGG TGAAATGGTAGACACGCGAG 50.0 50.0 58.4 58.0 828 bp 1.0 77.2
ycf15-trnL-CAA_p5 GCCAAAGTCACTGAATGGGT TGGTGAAATGGTAGACACGC 50.0 50.0 58.4 58.2 830 bp 1.0 75.7