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psaJ-rpl33

LSC · 65358-65811 · 454 bp

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Pi 0.00411

MarkerSeek score 51.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 5
pi: 0.00411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCCCGGATGCTTTGTCATT	GAAATCCCCGTCGATTCCTC	45.0	55.0	57.8	58.1	558-579 bp	1.0	81.9
psaJ-rpl33_p2	CGGATGCTTTGTCATTCCCT	GAAATCCCCGTCGATTCCTC	50.0	55.0	58.2	58.1	554-575 bp	1.0	81.7
psaJ-rpl33_p3	TTCCCGGATGCTTTGTCATT	GGCACCTTTTCGAACACAAC	45.0	50.0	57.8	58.2	535-556 bp	1.0	81.7
psaJ-rpl33_p4	TCCCGGATGCTTTGTCATTC	GAAATCCCCGTCGATTCCTC	50.0	55.0	58.2	58.1	557-578 bp	1.0	81.6
psaJ-rpl33_p5	CGGATGCTTTGTCATTCCCT	GGCACCTTTTCGAACACAAC	50.0	50.0	58.2	58.2	531-552 bp	1.0	81.5