Hotspot

trnT-UGU-trnL-UAA

LSC · 46329-46936 · 608 bp

Back to Hordeum

Pi 0.008
MarkerSeek score 48.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
608
variable_sites
11
pi
0.008
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 709-724 bp 1.0 81.1
trnT-UGU-trnL-UAA_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 701-716 bp 1.0 80.8
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 679-694 bp 1.0 80.3
trnT-UGU-trnL-UAA_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 699-714 bp 1.0 79.2
trnT-UGU-trnL-UAA_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 708-723 bp 1.0 78.9