Hotspot

trnG-GCC-trnfM-CAU

LSC · 12197-12642 · 446 bp

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Pi 0.0112

MarkerSeek score 51.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 12
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GTCGACCCAAGCGGATATAC	TCTGGTACTACAGAGCGGAG	55.0	55.0	57.9	58.0	644-646 bp	1.0	80.8
trnG-GCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	TCTGGTACTACAGAGCGGAG	50.0	55.0	58.1	58.0	604-606 bp	1.0	80.7
trnG-GCC-trnfM-CAU_p3	TATACCGTAGCGAGCGTAGT	TCTGGTACTACAGAGCGGAG	50.0	55.0	57.8	58.0	613-615 bp	1.0	80.1
trnG-GCC-trnfM-CAU_p4	CGTAGCGAGCGTAGTTCAAT	TCTGGTACTACAGAGCGGAG	50.0	55.0	58.2	58.0	608-610 bp	1.0	80.0
trnG-GCC-trnfM-CAU_p5	TAGCGAGCGTAGTTCAATGG	TCTGGTACTACAGAGCGGAG	50.0	55.0	57.8	58.0	606-608 bp	1.0	79.8