Hotspot

rps16-trnQ-UUG

LSC · 5538-6322 · 785 bp

Back to Hordeum

Pi 0.0381

MarkerSeek score 77.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 80
pi: 0.0381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	843-859 bp	1.0	89.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	854-870 bp	1.0	89.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	847-863 bp	1.0	88.7
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	45.0	45.0	58.1	57.8	912-928 bp	1.0	88.5
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.2	57.8	923-939 bp	1.0	87.9