Hotspot

psaC

SSC · 115429-115674 · 246 bp

Back to Hopea

Pi 0.294
MarkerSeek score 74.10
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
108
pi
0.294
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 CTTCCGCCAGCTGGATTAAT TGGTTAGGGCCCTTATGTGT 50.0 50.0 58.0 58.3 1029 bp 0.5 57.6
psaC_p2 CTTCCGCCAGCTGGATTAAT AGGGCCCTTATGTGTCTTGA 50.0 50.0 58.0 58.3 1024 bp 0.5 57.5
psaC_p3 ACCGTAGAACCCAATACCCA TGGTTAGGGCCCTTATGTGT 50.0 50.0 58.0 58.3 1397 bp 0.5 57.5
psaC_p4 CTTCCGCCAGCTGGATTAAT GGTTAGGGCCCTTATGTGTC 50.0 55.0 58.0 57.7 1028 bp 0.5 57.4
psaC_p5 ACCGTAGAACCCAATACCCA AGGGCCCTTATGTGTCTTGA 50.0 50.0 58.0 58.3 1392 bp 0.5 57.4