Hotspot

ndhI

SSC · 117318-117821 · 504 bp

Pi 0.314

MarkerSeek score 80.90

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 239
pi: 0.314
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AATAAGTAACTGGGCAGCGG	CAAGATGGACTTTCCCTCGG	50.0	55.0	58.0	58.0	1206-1208 bp	0.5	60.1
ndhI_p2	AATAAGTAACTGGGCAGCGG	TTCCCTAGCGGAATGTGAGA	50.0	50.0	58.0	58.1	1505-1507 bp	0.5	59.2
ndhI_p3	AATAAGTAACTGGGCAGCGG	AATGCGGATCCGGTCATTAG	50.0	50.0	58.0	57.8	1730-1732 bp	0.5	58.8
ndhI_p4	AATAAGTAACTGGGCAGCGG	TGGGTTTTGGGGTTGGAATT	50.0	45.0	58.0	57.8	1565-1567 bp	0.5	58.7
ndhI_p5	AATAAGTAACTGGGCAGCGG	ATTTGTGGCGTCAACCCATA	50.0	45.0	58.0	57.8	1548-1550 bp	0.5	58.6