Hotspot

ndhH

SSC · 120069-121250 · 1182 bp

Pi 0.275

MarkerSeek score 80.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 488
pi: 0.275
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGCTCGCAAACCACCTAAAA	AACGAAGAAAACAGGGGGTC	45.0	50.0	58.0	58.0	2009 bp	0.5	60.1
ndhH_p2	CAGTTTTGTCCCATCCGCTA	AACGAAGAAAACAGGGGGTC	50.0	50.0	57.9	58.0	1763 bp	0.5	59.5
ndhH_p3	AGCTCGCAAACCACCTAAAA	CCATTTCTTTGTAGCCCGGT	45.0	50.0	58.0	58.2	2237-2251 bp	0.5	59.4
ndhH_p4	CCATCCGCTAGAGCTTGAAG	AACGAAGAAAACAGGGGGTC	55.0	50.0	58.1	58.0	1753 bp	0.5	59.2
ndhH_p5	GCGTATTCAGGACCAGTACG	AACGAAGAAAACAGGGGGTC	55.0	50.0	58.2	58.0	1717 bp	0.5	59.0