Hotspot

ndhE

SSC · 115891-116196 · 306 bp

Pi 0.278

MarkerSeek score 75.50

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 128
pi: 0.278
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTCGAGTCGTTTCATGCCAT	CCGCTGCCCAGTTACTTATT	45.0	50.0	57.9	58.0	1389 bp	0.5	59.5
ndhE_p2	CTGTTCTTGGAGCAGAAGCA	CCGCTGCCCAGTTACTTATT	50.0	50.0	58.1	58.0	1299 bp	0.5	59.4
ndhE_p3	TCTGTTCTTGGAGCAGAAGC	CCGCTGCCCAGTTACTTATT	50.0	50.0	57.8	58.0	1300 bp	0.5	58.9
ndhE_p4	GGGTTTTGGGGAACGTATCA	CCGCTGCCCAGTTACTTATT	50.0	50.0	57.8	58.0	1426 bp	0.5	58.6
ndhE_p5	TTCGAGTCGTTTCATGCCAT	TTGCTGTGGCTCGTCAATAA	45.0	45.0	57.9	57.8	1053 bp	0.5	58.5