Hotspot

ndhD

SSC · 113785-115287 · 1503 bp

Pi 0.286

MarkerSeek score 81.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 645
pi: 0.286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCAGTCTGGGCTAATGAGTC	TGATCCCTTGGGATGGATGT	55.0	50.0	58.1	58.1	2306 bp	0.5	59.1
ndhD_p2	GCAGTCTGGGCTAATGAGTC	ATGGCATGAAACGACTCGAA	55.0	45.0	58.1	57.9	2182 bp	0.5	59.1
ndhD_p3	GCAGTCTGGGCTAATGAGTC	TGCTTCTGCTCCAAGAACAG	55.0	50.0	58.1	58.1	2272 bp	0.5	59.0
ndhD_p4	AGTTGGGACCCAAAGGAAAC	TGATCCCTTGGGATGGATGT	50.0	50.0	57.9	58.1	2270 bp	0.5	58.9
ndhD_p5	GTTGGGACCCAAAGGAAACT	TGATCCCTTGGGATGGATGT	50.0	50.0	57.9	58.1	2269 bp	0.5	58.9