Hotspot

psbZ-trnG-UCC

LSC · 36326-36946 · 621 bp

Back to Hopea

Pi 0.0115
MarkerSeek score 71.30
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
621
variable_sites
14
pi
0.0115
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 CTTCTCCTGATGGTTGGGTG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 57.9 57.8 775-851 bp 1.0 81.8
psbZ-trnG-UCC_p2 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 778-854 bp 1.0 81.1
psbZ-trnG-UCC_p3 TTCTCCTGATGGTTGGGTGA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 774-850 bp 1.0 80.7
psbZ-trnG-UCC_p4 CTTCTCCTGATGGTTGGGTG TAAATACATTGCCTGGGCGG 55.0 50.0 57.9 58.3 814-890 bp 1.0 80.4
psbZ-trnG-UCC_p5 TCCTGATGGTTGGGTGAGTA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.7 57.8 771-847 bp 1.0 80.1