Hotspot

trnfM-CAU

LSC · 37430-37483 · 54 bp

Back to Homolepis

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label trnfM-CAU

Feature Metadata

label_name: trnfM-CAU
feature_type: tRNA
region: LSC
length_bp: 54
variable_sites: 1
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU_p1	AGGAATATAGAGCGGGTAGTGA	TATGGTAGAGGGGCGTATCC	45.5	55.0	57.6	57.8	142 bp	1.0	59.7
trnfM-CAU_p2	AGGAATATAGAGCGGGTAGTGA	TTATCCACAAGTCCCCTGCT	45.5	50.0	57.6	58.3	113 bp	1.0	59.0
trnfM-CAU_p3	AGGAATATAGAGCGGGTAGTGA	GCGTATCCAACCCCCTTTTA	45.5	50.0	57.6	57.6	130 bp	1.0	58.2
trnfM-CAU_p4	AGGAATATAGAGCGGGTAGTGA	TTTATCCACAAGTCCCCTGC	45.5	50.0	57.6	57.5	114 bp	1.0	57.6
trnfM-CAU_p5	GGAATATAGAGCGGGTAGTGAG	TATGGTAGAGGGGCGTATCC	50.0	55.0	57.3	57.8	141 bp	1.0	57.1