Hotspot

matK

LSC · 1683-3224 · 1542 bp

Back to Homolepis

Pi 0.0013

MarkerSeek score 59.40

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1542
variable_sites: 2
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAGAATGGAAAATGTGCAAGT	GCAACACCTTGTTCTGACCA	36.4	50.0	56.8	58.6	1717 bp	1.0	49.5
matK_p2	TCAGAATGGAAAATGTGCAAGT	TGCAACACCTTGTTCTGACC	36.4	50.0	56.8	58.6	1718 bp	1.0	49.5
matK_p3	TCAGAATGGAAAATGTGCAAGTT	GCAACACCTTGTTCTGACCA	34.8	50.0	57.5	58.6	1717 bp	1.0	46.9
matK_p4	TCAGAATGGAAAATGTGCAAGTT	TGCAACACCTTGTTCTGACC	34.8	50.0	57.5	58.6	1718 bp	1.0	46.9
matK_p5	TTCAGAATGGAAAATGTGCAAGT	GCAACACCTTGTTCTGACCA	34.8	50.0	57.5	58.6	1718 bp	1.0	46.9