Hotspot

atpB-rbcL

LSC · 54954-55762 · 809 bp

Back to Homolepis

Pi 0

MarkerSeek score 59.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	985-991 bp	1.0	77.8
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	984-990 bp	1.0	77.8
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	982-988 bp	1.0	77.5
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	981-987 bp	1.0	77.5
atpB-rbcL_p5	TATCCAGCACGGGTCCAATA	CGTACTCCGGGGTGTAGTAA	50.0	55.0	58.2	58.2	988-994 bp	1.0	77.0