Hotspot

psbM-petN

LSC · 17998-18779 · 782 bp

Back to Homolepis

Pi 0.00128

MarkerSeek score 59.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 1
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	889 bp	1.0	68.5
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GGCTGCTTTAATGGTGGTCT	40.0	50.0	56.7	58.2	873 bp	1.0	68.4
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.7	58.3	833 bp	1.0	67.5
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	56.7	58.4	832 bp	1.0	66.7
psbM-petN_p5	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	887 bp	1.0	66.6