Hotspot

trnD-GUC-psbM

LSC · 16835-17892 · 1058 bp

Back to Homolepis

Pi 0.00284

MarkerSeek score 60.70

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 3
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	1221-1223 bp	1.0	66.2
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	1223-1225 bp	1.0	64.3
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	1222-1224 bp	1.0	64.3
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.5	57.4	1221-1223 bp	1.0	64.1
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	TTTGGCTGGCTGTTTTTACA	50.0	40.0	58.5	56.3	1217-1219 bp	1.0	63.4