Hotspot

rps16-trnQ-UUG

LSC · 5603-6783 · 1181 bp

Back to Homolepis

Pi 0.000848

MarkerSeek score 59.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1181
variable_sites: 1
pi: 0.000848
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1253-1255 bp	1.0	78.5
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1264-1266 bp	1.0	77.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1257-1259 bp	1.0	77.4
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1268-1270 bp	1.0	76.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AAACGAAATGAAATGGGGCG	45.0	45.0	58.1	57.6	1314-1316 bp	1.0	76.2