Hotspot

atpF

LSC · 14757-16147 · 1391 bp

Back to Homalomena

Pi 0.000726

MarkerSeek score 60.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1391
variable_sites: 1
pi: 0.000726
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	ATCAACCAAAAAGGGGAGGG	45.0	50.0	57.4	57.7	1565-1576 bp	1.0	73.2
atpF_p2	ACTAATTTCGTCGGCTCGAA	GGAGGGCGAAGTGATACAAA	45.0	50.0	57.4	57.6	1551-1562 bp	1.0	72.6
atpF_p3	AATTTCGTCGGCTCGAATGG	ATCAACCAAAAAGGGGAGGG	50.0	50.0	59.0	57.7	1562-1573 bp	1.0	70.4
atpF_p4	AATTTCGTCGGCTCGAATGG	GGAGGGCGAAGTGATACAAA	50.0	50.0	59.0	57.6	1548-1559 bp	1.0	69.8
atpF_p5	ACTAATTTCGTCGGCTCGAAT	ATCAACCAAAAAGGGGAGGG	42.9	50.0	57.8	57.7	1565-1576 bp	1.0	69.0