Hotspot

psaC-ndhE

SSC · 126456-127090 · 635 bp

Back to Homalomena

Pi 0

MarkerSeek score 63.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 635
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AAAACATCTGTAGGGCAGGC	CGTAACAGAAAATCAACGCGT	50.0	42.9	58.2	57.8	730-763 bp	1.0	72.9
psaC-ndhE_p2	AAAACATCTGTAGGGCAGGC	GCCGAAGCAGCTATTGGTTT	50.0	50.0	58.2	59.2	772-805 bp	1.0	72.6
psaC-ndhE_p3	AAAACATCTGTAGGGCAGGC	CGAAGCAGCTATTGGTTTAGC	50.0	47.6	58.2	57.7	770-803 bp	1.0	71.5
psaC-ndhE_p4	TGCTTAGCTTTACATCCCGG	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.7	57.8	766-799 bp	1.0	71.5
psaC-ndhE_p5	TGCTTAGCTTTACATCCCGG	GCCGAAGCAGCTATTGGTTT	50.0	50.0	57.7	59.2	808-841 bp	1.0	71.1