Hotspot

rpl32-trnL-UAG

SSC · 122592-123231 · 640 bp

Back to Homalomena

Pi 0.00469

MarkerSeek score 61.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 640
variable_sites: 3
pi: 0.00469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTTGGCTGCGCTAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	775-783 bp	1.0	77.1
rpl32-trnL-UAG_p2	GGGATATTTGGCTGCGCTAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.4	58.0	776-784 bp	1.0	77.1
rpl32-trnL-UAG_p3	GGGATATTTGGCTGCGCTAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	785-793 bp	1.0	76.1
rpl32-trnL-UAG_p4	GGGATATTTGGCTGCGCTAA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.4	57.8	784-792 bp	1.0	75.6
rpl32-trnL-UAG_p5	GGGATATTTGGCTGCGCTAA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.4	57.8	783-791 bp	1.0	75.6