Hotspot

psbT-psbN

LSC · 82497-82570 · 74 bp

Back to Homalomena

Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCTCCTAAGGTTCCGACTAA	GAGACCCATTCGAGGAACAC	50.0	55.0	55.7	58.0	136 bp	1.0	61.9
psbT-psbN_p2	CCTCCTAAGGTTCCGACTAA	TCGCCATCTCCATATCTGGT	50.0	50.0	55.7	58.0	220 bp	1.0	61.6
psbT-psbN_p3	CCTCCTAAGGTTCCGACTAA	AGAGACCCATTCGAGGAACA	50.0	50.0	55.7	58.1	137 bp	1.0	61.5
psbT-psbN_p4	CCTCCTAAGGTTCCGACTAAA	GAGACCCATTCGAGGAACAC	47.6	55.0	56.5	58.0	136 bp	1.0	60.2
psbT-psbN_p5	CCTCCTAAGGTTCCGACTAA	ACCTTAGTCGCCATCTCCAT	50.0	50.0	55.7	58.2	227 bp	1.0	60.1