Hotspot

psbZ-trnG-GCC

LSC · 40735-41324 · 590 bp

Back to Homalomena

Pi 0.0102

MarkerSeek score 65.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 6
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTTCCGATGGATGG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.0	58.1	755-758 bp	1.0	80.2
psbZ-trnG-GCC_p2	TTGCTTCTTCCGATGGATGG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.0	58.1	756-759 bp	1.0	80.2
psbZ-trnG-GCC_p3	TTGCTTCTTCCGATGGATGG	TACTTCACCATGGGCGGATA	50.0	50.0	58.0	58.2	780-783 bp	1.0	79.1
psbZ-trnG-GCC_p4	TTGCTTCTTCCGATGGATGG	ACATTACTTCACCATGGGCG	50.0	50.0	58.0	58.2	784-787 bp	1.0	78.7
psbZ-trnG-GCC_p5	GCTTCTTCCGATGGATGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.4	58.1	753-756 bp	1.0	77.3