Hotspot

trnS-GCU-trnG-UCC

LSC · 10013-12645 · 2633 bp

Back to Homalomena

Pi 0.00231

MarkerSeek score 52.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 2633
variable_sites: 6
pi: 0.00231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	CATCGTTAGCTTGGAAGGCT	45.0	50.0	58.0	58.0	2783-2946 bp	1.0	80.7
trnS-GCU-trnG-UCC_p2	GTGGAGAGAGAGGGATTCGA	CATCGTTAGCTTGGAAGGCT	55.0	50.0	57.9	58.0	2832-2995 bp	1.0	80.5
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	CGTTAGCTTGGAAGGCTAGG	45.0	55.0	58.0	58.1	2780-2943 bp	1.0	80.5
trnS-GCU-trnG-UCC_p4	AGTGGAGAGAGAGGGATTCG	CATCGTTAGCTTGGAAGGCT	55.0	50.0	57.9	58.0	2833-2996 bp	1.0	80.4
trnS-GCU-trnG-UCC_p5	GTGGAGAGAGAGGGATTCGA	CGTTAGCTTGGAAGGCTAGG	55.0	55.0	57.9	58.1	2829-2992 bp	1.0	80.3