Hotspot

ndhF

SSC · 112369-114648 · 2280 bp

Back to Homalium

Pi 0.00819

MarkerSeek score 48.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2280
variable_sites: 28
pi: 0.00819
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GTGGGCTCTATCGTGGAAAA	AAGTTTCGAGTTCTGTCCCG	50.0	50.0	57.6	57.8	2910-2934 bp	1.0	78.2
ndhF_p2	AGTGGGCTCTATCGTGGAAA	AAGTTTCGAGTTCTGTCCCG	50.0	50.0	58.4	57.8	2911-2935 bp	1.0	77.9
ndhF_p3	GTGGGCTCTATCGTGGAAAA	GTTCTGTCCCGGACTTCAAA	50.0	50.0	57.6	57.8	2901-2925 bp	1.0	77.4
ndhF_p4	AGTGGGCTCTATCGTGGAAA	GTTCTGTCCCGGACTTCAAA	50.0	50.0	58.4	57.8	2902-2926 bp	1.0	77.1
ndhF_p5	TTTGAGTGGGCTCTATCGTG	AAGTTTCGAGTTCTGTCCCG	50.0	50.0	57.3	57.8	2915-2939 bp	1.0	76.0