Hotspot

trnN-trnR

IRa · 131050-131720 · 671 bp

Back to Homalium

Pi 0.00399

MarkerSeek score 65.50

Primer available yes

Label trnN-trnR

Feature Metadata

label_name: trnN-trnR
feature_type: igs
region: IRa
length_bp: 671
variable_sites: 4
pi: 0.00399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-trnR_p1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	825-850 bp	1.0	82.1
trnN-trnR_p2	CCGTTGTTCCTCAGTAGCTC	TAGGGTATTCCGGGGAACAG	55.0	55.0	58.0	58.2	840-865 bp	1.0	81.1
trnN-trnR_p3	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	789-814 bp	1.0	81.1
trnN-trnR_p4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	789-814 bp	1.0	81.1
trnN-trnR_p5	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	776-801 bp	1.0	81.1