Hotspot

ycf1-ndhF

SSC · 112275-112368 · 94 bp

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Pi 0.119

MarkerSeek score 67.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 10
pi: 0.119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GTGGGCTCTATCGTGGAAAA	ATATGTAGGAGGTGGTCGCA	50.0	50.0	57.6	57.9	367-415 bp	1.0	82.9
ycf1-ndhF_p2	GTGGGCTCTATCGTGGAAAA	TATGTAGGAGGTGGTCGCAT	50.0	50.0	57.6	57.9	366-414 bp	1.0	82.9
ycf1-ndhF_p3	GTGGGCTCTATCGTGGAAAA	GGAGTCGGCTTTACGAGTTT	50.0	50.0	57.6	57.9	408-456 bp	1.0	82.8
ycf1-ndhF_p4	GTGGGCTCTATCGTGGAAAA	GAGGTGGTCGCATCTCTTTT	50.0	50.0	57.6	57.9	359-407 bp	1.0	82.8
ycf1-ndhF_p5	AGTGGGCTCTATCGTGGAAA	GGAGTCGGCTTTACGAGTTT	50.0	50.0	58.4	57.9	409-457 bp	1.0	82.6